THP-1 Macrophages Limit Neutrophil Transendothelial Migration in a Model Infection.

ABSTRACT:

Introduction

Dysregulated neutrophil function plays a significant role in the pathology of infections, cancer, cardiovascular diseases, and autoimmune disorders. Neutrophil activity is influenced by various cell populations, including macrophages, which are crucial regulators. However, the exact role of human macrophages in controlling neutrophil function remains unclear due to a scarcity of studies utilizing human cells in physiologically relevant models.

Methods

We adapted our "Infection-on-a-Chip" microfluidic device to incorporate macrophages within the collagen extracellular matrix, allowing for the study of interactions between human neutrophils and macrophages in a context that mimics in vivo conditions. The integration of THP-1 macrophages was optimized and their eff

SUBMITTER: Ignes-Romeu A 

PROVIDER: S-EPMC11450111 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature