Light-Activatable, Cell-Type Specific Labeling of the Nascent Proteome.

ABSTRACT: Elucidating the mechanisms by which protein synthesis contributes to complex biological processes has remained a challenging endeavor. This is particularly true in the field of neuroscience, where multiple, tightly regulated periods of new protein synthesis in different cell-types are thought to facilitate intricate neurological functions, such as memory formation. Current methods for labeling the de novo proteome have lacked the spatial and temporal resolution to accurately discriminate these overlapping and often competing windows of mRNA translation. To address this technological limitation, here we describe a novel, light-inducible specific method for labeling newly synthesized proteins within a targeted cell-type.By developing Opto-ANL, a photocaged version of the nonendogenous

SUBMITTER: Evans HT 

PROVIDER: S-EPMC11450754 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature