Automated Assignment of ¹⁵N And ¹³C Enrichment Levels in Doubly-Labeled Proteins.

ABSTRACT: Uniform enrichment of ¹⁵N and ¹³C in proteins is commonly employed for 2D heteronuclear NMR measurements of the three-dimensional protein structure. Achieving a high degree of enrichment of both elements is important for obtaining high quality data. Uniform labeling of proteins and glycoproteins expressed in higher organisms (yeast or mammalian cell lines) is more challenging than expression in Escherichia coli, a prokaryote that grows on simple, chemically defined media but does not provide appropriate eukaryotic modifications. It is difficult to achieve complete incorporation of both heavy isotopes, and quality control measures are important for quantitating the level of their enrichment. Mass spectrometry measurements of the isotopic distribution of the inta

SUBMITTER: Roberts ET 

PROVIDER: S-EPMC11450805 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature