Truncated M13 phage for smart detection of E. coli under dark field.

ABSTRACT:

Background

The urgent need for affordable and rapid detection methodologies for foodborne pathogens, particularly Escherichia coli (E. coli), highlights the importance of developing efficient and widely accessible diagnostic systems. Dark field microscopy, although effective, requires specific isolation of the target bacteria which can be hindered by the high cost of producing specialized antibodies. Alternatively, M13 bacteriophage, which naturally targets E. coli, offers a cost-efficient option with well-established techniques for its display and modification. Nevertheless, its filamentous structure with a large length-diameter ratio contributes to nonspecific binding and low separation efficiency, posing significant challenges. Consequently, refining M13 phage methodologies and

SUBMITTER: Yuan J 

PROVIDER: S-EPMC11451008 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature