Human-to-Anopheles dirus mosquito transmission of the anthropozoonotic malaria parasite, Plasmodium knowlesi.

ABSTRACT:

Background

Plasmodium knowlesi, identified as the fifth human malaria parasite, has rapidly spread across various Southeast Asian countries, yet uncertainties persist regarding its human-mosquito-human transmission. Therefore, this study aims to explore the transmission potential of P. knowlesi from human blood to mosquitoes.

Methods

A direct membrane-feeding assay was conducted by infecting laboratory-reared female Anopheles dirus mosquitoes with P. knowlesi-infected human blood from a single patient presenting with febrile malaria. Mosquitoes were dissected 7 days post-infection under a stereomicroscope to detect oocysts in the midgut, stained with mercurochrome. Salivary glands were examined 14 days post-infection for the presence of sporozoites. Malaria diagnosis employe

SUBMITTER: Kumpitak C 

PROVIDER: S-EPMC11451161 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature