Structural perturbation of chromatin domains with multiple developmental regulators can severely impact gene regulation and development.

ABSTRACT: Chromatin domain boundaries delimited by CTCF motifs can restrict the range of enhancer action. However, disruption of domain structure often results in mild gene dysregulation and thus predicting the impact of boundary rearrangements on animal development remains challenging. Here, we tested whether structural perturbation of a chromatin domain with multiple developmental regulators can result in more acute gene dysregulation and severe developmental phenotypes. We targeted clusters of CTCF motifs in a domain of the mouse genome containing three FGF ligand genes-Fgf3, Fgf4, and Fgf15-that regulate several developmental processes. Deletion of the 23.9kb cluster that defines the centromeric boundary of this domain resulted in ectopic interactions of the FGF genes with e

SUBMITTER: Chakraborty S 

PROVIDER: S-EPMC11451586 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature