Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells.

ABSTRACT: Acquisition of specific cell shapes and morphologies is a central component of cell fate transitions. Although signaling circuits and gene regulatory networks that regulate pluripotent stem cell differentiation have been intensely studied, how these networks are integrated in space and time with morphological transitions and mechanical deformations to control state transitions remains a fundamental open question. Here, we focus on two distinct models of pluripotency, primed pluripotent stem cells and pre-implantation inner cell mass cells of human embryos to discover that cell fate transitions associate with rapid changes in nuclear shape and volume which collectively alter the nuclear mechanophenotype. Mechanistic studies in human induced pluripotent stem cells further reveal that these p

SUBMITTER: McCreery KP 

PROVIDER: S-EPMC11451594 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature