Structure-guided assembly of an influenza spike nanobicelle vaccine provides pan H1 intranasal protection.

ABSTRACT: Development of intranasal vaccines for respiratory viruses has gained popularity. However, currently only a live-attenuated influenza vaccine is FDA-approved for intranasal administration. Here, we focused on influenza virus as it circulates seasonally, has pandemic potential, and has vaccine formulations that present hemagglutinin (HA) in different structural arrangements. These display differences have not been correlated with induction of pan-H1 antibodies or shown to provide intranasal protection. Using electron microscopy, biochemistry and animal studies, we identified HA complexes arranged as lipid discs with multiple trimeric HAs displayed along the perimeter, termed spike nanobicelles (SNB). We utilized a structure-guided approach to synthesize in vitro assembled spiked nanobicelle

SUBMITTER: Myers ML 

PROVIDER: S-EPMC11451756 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature