Comprehensive in silico analyses of fifty-one uncharacterized proteins from Vibrio cholerae.

ABSTRACT: Due to the rise of multidrug-resistant strains of Vibrio cholerae and the recent cholera outbreaks in African and Asian nations, it is imperative to identify novel therapeutic targets and possible vaccine candidates. In this regard, this work primarily aims to identify and characterize new antigenic molecules using comparative RNA sequencing data and label-free proteomics data, carried out with essential GTPase cgtA knockdown and wild-type strain of V. cholerae. We identified hitherto 51 characterized proteins from high-throughput RNA-sequencing and proteomics data. This work involved the assessment of their physicochemical characteristics, subcellular localization, solubility, structures, and functional annotations. In addition, the immunoinformatic and reverse vaccinology technique was u

SUBMITTER: Basu Mallick S 

PROVIDER: S-EPMC11452002 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature