A deficiency screen identifies genomic regions critical for sperm head-tail connection.

ABSTRACT: A stable connection between the sperm head and tail is critical for fertility in species with flagellated sperm. The head-tail coupling apparatus (HTCA) serves as the critical link between the nucleus (head) and the axoneme (tail) via the centriole. To identify regions of the Drosophila melanogaster genome that contain genetic elements that influence HTCA formation, we undertook a two part screen using the Drosophila deficiency (Df) kit. For this screen, we utilized a sensitized genetic background that overexpresses the pericentriolar material regulatory protein Pericentrin-Like Protein (PLP). We had previously shown that PLP overexpression (PLP^OE) disrupts the head-tail connection in some spermatids, but not to a degree sufficient to reduce fertility. In the first

SUBMITTER: Galletta BJ 

PROVIDER: S-EPMC11452195 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature