Real-time visualisation of developing chick embryos cultured in transparent plastic films from the blastoderm stage until hatching.

ABSTRACT: Real-time visualisation of chick embryo development occurring inside an opaque eggshell is a major goal of developmental biology. This goal was partially achieved when 3-day-old embryos recovered from fertilised shelled eggs, preincubated in a conventional incubator, hatched after the egg contents had been transferred into shell-less embryo culture systems constructed of transparent plastic film placed in a plastic cup. However, the hatchable shell-less embryo culture systems described thus far cannot be used to visualise the dynamic morphological changes associated with the emergence of major organ systems during the first 3 days of embryo development, as the blastoderm area needs to be covered with an opaque membrane. Here, we present the development of a chick embryo culture system that

SUBMITTER: Obara K 

PROVIDER: S-EPMC11452206 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature