Developmental assembly of multi-component polymer systems through interconnected synthetic gene networks in vitro.

ABSTRACT: Living cells regulate the dynamics of developmental events through interconnected signaling systems that activate and deactivate inert precursors. This suggests that similarly, synthetic biomaterials could be designed to develop over time by using chemical reaction networks to regulate the availability of assembling components. Here we demonstrate how the sequential activation or deactivation of distinct DNA building blocks can be modularly coordinated to form distinct populations of self-assembling polymers using a transcriptional signaling cascade of synthetic genes. Our building blocks are DNA tiles that polymerize into nanotubes, and whose assembly can be controlled by RNA molecules produced by synthetic genes that target the tile interaction domains. To achieve different RNA productio

SUBMITTER: Sorrentino D 

PROVIDER: S-EPMC11452209 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature