Characteristics of blood-brain barrier heterogeneity between brain regions revealed by profiling vascular and perivascular cells.

ABSTRACT: The blood-brain barrier (BBB) protects the brain and maintains neuronal homeostasis. BBB properties can vary between brain regions to support regional functions, yet how BBB heterogeneity occurs is poorly understood. Here, we used single-cell and spatial transcriptomics to compare the mouse median eminence, one of the circumventricular organs that has naturally leaky blood vessels, with the cortex. We identified hundreds of molecular differences in endothelial cells (ECs) and perivascular cells, including astrocytes, pericytes and fibroblasts. Using electron microscopy and an aqueous-based tissue-clearing method, we revealed distinct anatomical specializations and interaction patterns of ECs and perivascular cells in these regions. Finally, we identified candidate regionally enriched EC-pe

SUBMITTER: Pfau SJ 

PROVIDER: S-EPMC11452347 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature