Super-resolved analysis of colocalization between replication and transcription along the cell cycle in a model of oncogene activation.

ABSTRACT: To understand how oncogenes affect genome organization, it is essential to visualize fundamental processes such as DNA replication and transcription at high resolution in intact cells. At the same time, it is important to determine the progression of the cell along the cell cycle, as cell cycle regulation is crucial for the control of cell proliferation and oncogenesis. Here, we present a super-resolution imaging-based method to analyze single cell nuclei sorted according to specific phases of the cell cycle. The sorting is based on the evaluation of the number and the intensity of pixels in the replication foci image and the colocalization analysis is based on image cross-correlation spectroscopy (ICCS). We evaluate the colocalization between replication and transcription, at different ce

SUBMITTER: Privitera AP 

PROVIDER: S-EPMC11452374 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature