Plateau depolarizations in spontaneously active neurons detected by calcium or voltage imaging.

ABSTRACT: In calcium imaging studies, Ca²⁺ transients are commonly interpreted as neuronal action potentials (APs). However, our findings demonstrate that robust optical Ca²⁺ transients primarily stem from complex "AP-Plateaus", while simple APs lacking underlying depolarization envelopes produce much weaker photonic signatures. Under challenging in vivo conditions, these "AP-Plateaus" are likely to surpass noise levels, thus dominating the Ca²⁺ recordings. In spontaneously active neuronal culture, optical Ca²⁺ transients (OGB1-AM, GCaMP6f) exhibited approximately tenfold greater amplitude and twofold longer half-width compared to optical voltage transients (ArcLightD). The amplitude of the ArcLightD signal exhibited a strong correlation with the duration

SUBMITTER: Milicevic KD 

PROVIDER: S-EPMC11452489 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature