Two-photon all-optical neurophysiology for the dissection of larval zebrafish brain functional and effective connectivity.

ABSTRACT: One of the most audacious goals of modern neuroscience is unraveling the complex web of causal relations underlying the activity of neuronal populations on a whole-brain scale. This endeavor, which was prohibitive only a couple of decades ago, has recently become within reach owing to the advancements in optical methods and the advent of genetically encoded indicators/actuators. These techniques, applied to the translucent larval zebrafish have enabled recording and manipulation of the activity of extensive neuronal populations spanning the entire vertebrate brain. Here, we present a custom two-photon optical system that couples light-sheet imaging and 3D excitation with acousto-optic deflectors for simultaneous high-speed volumetric recording and optogenetic stimulation. By employing a ze

SUBMITTER: Turrini L 

PROVIDER: S-EPMC11452506 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature