Biofunctionalized dissolvable hydrogel microbeads enable efficient characterization of native protein complexes.

ABSTRACT: The characterization of protein complex is vital for unraveling biological mechanisms in various life processes. Despite advancements in biophysical tools, the capture of non-covalent complexes and deciphering of their biochemical composition continue to present challenges for low-input samples. Here we introduce SNAP-MS, a Stationary-phase-dissolvable Native Affinity Purification and Mass Spectrometric characterization strategy. It allows for highly efficient purification and characterization from inputs at the pico-mole level. SNAP-MS replaces traditional elution with matrix dissolving during the recovery of captured targets, enabling the use of high-affinity bait-target pairs and eliminates interstitial voids. The purified intact protein complexes are compatible with native MS, which pr

SUBMITTER: Shao X 

PROVIDER: S-EPMC11452662 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature