Cryo-EM investigation of ryanodine receptor type 3.

ABSTRACT: Ryanodine Receptor isoform 3 (RyR3) is a large ion channel found in the endoplasmic reticulum membrane of many different cell types. Within the hippocampal region of the brain, it is found in dendritic spines and regulates synaptic plasticity. It controls myogenic tone in arteries and is upregulated in skeletal muscle in early development. RyR3 has a unique functional profile with a very high sensitivity to activating ligands, enabling high gain in Ca²⁺-induced Ca²⁺ release. Here we solve high-resolution cryo-EM structures of RyR3 in non-activating and activating conditions, revealing structural transitions that occur during channel opening. Addition of activating ligands yields only open channels, indicating an intrinsically high open probability under these conditio

SUBMITTER: Chen YS 

PROVIDER: S-EPMC11452665 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature