ABSTRACT: The complete mitochondrial genomes of Tipula (Pterelachisus) cinereocincta mesacantha Alexander, 1934, T. (P.) legalis Alexander, 1933, T. (P.) varipennis Meigen, 1818, and T. (P.) yasumatsuana Alexander, 1954 are reported, three of them being sequenced for the first time. The mitochondrial genome lengths of the four species are 15,907 bp, 15,625 bp, 15,772 bp, and 15,735 bp, respectively. All genomes exhibit a high AT base composition, with A + T content of 76.7%, 75.0%, 77.8%, and 75.4%, respectively. The newly reported mitogenomes herein show a general similarity in overall structure, gene order, base composition, and nucleotide content to those of the previously studied species within the family Tipulidae. Phylogenetic analyses were conducted to investigate the relationships within Tip