Protocol for mapping cell lineage and cell-type identity of clonally-related cells in situ using MADM-CloneSeq.

ABSTRACT: The lineage relationship of clonally-related cells offers important insights into the ontogeny and cytoarchitecture of the brain in health and disease. Here, we provide a protocol to concurrently assess cell lineage relationship and cell-type identity among clonally-related cells in situ. We first describe the preparation and screening of acute brain slices containing clonally-related cells labeled using mosaic analysis with double markers (MADM). We then outline steps to collect RNA from individual cells for downstream applications and cell-type identification using RNA sequencing. For complete details on the use and execution of this protocol, please refer to Cheung et al.¹.

SUBMITTER: Cheung G 

PROVIDER: S-EPMC11452916 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature