Production of α-ketoisovalerate with whey powder by systemic metabolic engineering of Klebsiella oxytoca.

ABSTRACT:

Background

Whey, which has high biochemical oxygen demand and chemical oxygen demand, is mass-produced as a major by-product of the dairying industry. Microbial fermentation using whey as the carbon source may convert this potential pollutant into value-added products. This study investigated the potential of using whey powder to produce α-ketoisovalerate, an important platform chemical.

Results

Klebsiella oxytoca VKO-9, an efficient L-valine producing strain belonging to Risk Group 1 organism, was selected for the production of α-ketoisovalerate. The leucine dehydrogenase and branched-chain α-keto acid dehydrogenase, which catalyzed the reductive amination and oxidative decarboxylation of α-ketoisovalerate, respectively, were inactivated to enhance the accumulation of α-ket

SUBMITTER: Sun W 

PROVIDER: S-EPMC11452931 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature