Adaptation of the protein misfolding cyclic amplification (PMCA) technique for the screening of anti-prion compounds.

ABSTRACT: Prion diseases result from the misfolding of the physiological prion protein (PrP^C) to a pathogenic conformation (PrP^Sc). Compelling evidence indicates that prevention and/or reduction of PrP^Sc replication are promising therapeutic strategies against prion diseases. However, the existence of different PrP^Sc conformations (or strains) associated with disease represents a major problem when identifying anti-prion compounds. Efforts to identify strain-specific anti-prion molecules are limited by the lack of biologically relevant high-throughput screening platforms to interrogate compound libraries. Here, we describe adaptations to the protein misfolding cyclic amplification (PMCA) technology (able to faithfully replicate PrP^Sc strains) th

SUBMITTER: Do K 

PROVIDER: S-EPMC11453167 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature