Expression of a novel NaD1 recombinant antimicrobial peptide enhances antifungal and insecticidal activities.

ABSTRACT: This study aimed to increase the antifungal and insecticidal activities of NaD1, as an antimicrobial peptides (AMP), by improving its interaction with the fungal cell wall and chitin monomeric units in insect midguts. Hence, the chitin-binding domains (CBDs) of wheat germ agglutinin protein (WGA) were fused to either N- or C-terminus of NaD1 generating transgenic Nicotiana tabacum hairy roots (HRs). Molecular assessments confirmed the integration of NaD1 transgenes, their transcription and production of recombinant peptides in the HR lines. Total protein of (CBD)₄-NaD1 and NaD1-(CBD)₄ transgenic lines inhibited the growth of Pyricularia oryzae mycelium, suggesting that fusion of CBD to NaD1 can increase NaD1 half-life, leading to higher affinity toward cell wall chiti

SUBMITTER: Royan S 

PROVIDER: S-EPMC11455875 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature