Combining human liver ECM with topographically featured electrospun scaffolds for engineering hepatic microenvironment.

ABSTRACT: Liver disease cases are rapidly expanding worldwide, and transplantation remains the only effective cure for end-stage disease. There is an increasing demand for developing potential drug treatments, and regenerative therapies using in-vitro culture platforms. Human decellularized extracellular matrix (dECM) is an appealing alternative to conventional animal tissues as it contains human-specific proteins and can serve as scaffolding materials. Herein we exploit this with human donor tissue from discarded liver which was not suitable for transplant using a synergistic approach to combining biological and topographical cues in electrospun materials as an in-vitro culture platform. To realise this, we developed a methodology for incorporating human liver dECM into electrospun polycaprolactone

SUBMITTER: Gao Y 

PROVIDER: S-EPMC11455933 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature