NPRL2 promotes TRIM16-mediated ubiquitination degradation of Galectin-3 to prevent CD8⁺T lymphocyte cuproptosis in glioma.

ABSTRACT:

Background

Our previous study found that tumor suppressor nitrogen permease regulator like-2(NPRL2) is frequently downregulated in glioma, leading to malignant growth. However, NPRL2-mediated crosstalk between tumor cells and immune cells remains unclear.

Methods

The regulatory effects of NPRL2 on tripartite motif-containing protein 16(TRIM16) dependent ubiquitination degradation of Galectin-3(Gal-3) were explored. The effects of Gal-3 on copper uptake, immunocompetence and cuproptosis were investigated in CD8⁺T lymphocytes(CD8⁺T cells). The ability of NPRL2 to protect CD8⁺T cells from Gal-3 damage was evaluated. Furthermore, the correlations among NPRL2, TRIM16, Gal-3 and CD8⁺T cell accumulation were analyzed in glioma clinical sp

SUBMITTER: Wang F 

PROVIDER: S-EPMC11456027 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature