Identification of age-associated microbial changes via long-read 16S sequencing.

ABSTRACT:

Background

Age-related gut microbial changes have been widely investigated over the past decade. Most of the previous age-related microbiome studies were conducted on the Western population, and the short-read sequencing (e.g., 16S V4 or V3-V4 region) was the most common microbiota profiling method. We evaluated the gut compositional differences using the long-read sequencing approach (i.e., PacBio sequencing targeting the full-length V1-V9 regions) to enable a deeper taxonomic resolution and better characterize the gut microbiome of Singaporeans from different age groups.

Results

A total of 83 research participants were included in this study. Although no significant differences were detected in alpha and beta diversity, our study demonstrated several bacterial taxa with ab

SUBMITTER: Toh KY 

PROVIDER: S-EPMC11456230 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature