Elucidating Thermothielavioides terrestris secretome changes for improved saccharification of mild steam-pretreated spruce.

ABSTRACT:

Background

The efficient use of softwood in biorefineries is hampered by its recalcitrance to enzymatic saccharification. In the present study, the fungus Thermothielavioides terrestris LPH172 was cultivated on three steam-pretreated spruce materials (STEX_180°C/auto, STEX_210°C/auto, and STEX_210°C/H2SO4), characterized by different hemicellulose content and structure, as well as on untreated biomass. The aim of the study was to map substrate-induced changes in the secretome of T. terrestris grown on differently treated spruce materials and to evaluate the hydrolytic efficiency of the secretome as supplement for a commercial enzyme mixture.

Results

The cultivation of T. terrestris was monitored by endo-cellulase, endo-xylanase, endo-mannanase,

SUBMITTER: Caputo F 

PROVIDER: S-EPMC11456254 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature