Single-cell RNA-sequencing of human eosinophils in allergic inflammation in the esophagus.

ABSTRACT:

Background

Eosinophils are elusive cells involved in allergic inflammation. Single-cell RNA-sequencing (scRNA-seq) is an emerging approach to deeply characterize cellular properties, heterogeneity, and functionality.

Objectives

We sought to comprehensively characterize the transcriptome and biological functions of human eosinophils at a site of severe allergic inflammation in the esophagus (ie, eosinophilic esophagitis [EoE]).

Methods

We employed a gravity-based scRNA-seq methodology to sequence blood eosinophils from patients with EoE and control individuals compared to a reanalyzed public scRNA-seq dataset of human esophageal eosinophils of EoE patients. We used flow cytometry, immunostaining, and a stimulation assay to verify mRNA findings.

Results

In total,

SUBMITTER: Ben-Baruch Morgenstern N 

PROVIDER: S-EPMC11456386 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature