CupAR negatively controls the key protein CupA in the carbon acquisition complex NDH-1MS in Synechocystis sp. PCC 6803.

ABSTRACT: The low CO₂-inducible NDH complex (NDH-1MS) plays a crucial role in the cyanobacterial CO₂-concentrating mechanism. However, the components in this complex and the regulation mechanism are still not completely understood. Using a mutant only with NDH-1MS as active Ci sequestration system, we identified a functional gene sll1736 named as cupAR (CupA Regulator). The cupAR deletion mutant, ΔcupAR, grew faster than the WT under high CO₂ (HC) condition, more evidently at low pH. The activities of O₂ evolution, CO₂ uptake，NDH-1, and the building up of a transthylakoid proton were stimulated in this mutant under HC conditions. The cupAR gene is cotranscribed with the NDH-1S operon (ndhF3-ndhD3-cupA) and encoded protein, which specifically sup

SUBMITTER: Liu J 

PROVIDER: S-EPMC11456788 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature