FcRider: a recombinant Fc nanoparticle with endogenous adjuvant activities for hybrid immunization.

ABSTRACT: Active immunization (vaccination) induces long-lasting immunity with memory, which takes weeks to months to develop. Passive immunization (transfer of neutralizing antibodies) provides immediate protection, yet with high cost and effects being comparatively short-lived. No currently approved adjuvants are compatible with formulations to combine active and passive immunizations, not to mention their huge disparities in administration routes and dosage. To solve this, we engineered the Fc fragment of human IgG1 into a hexamer nanoparticle and expressed its afucosylated form in Fut8-/- CHO cells, naming it "FcRider." FcRider is highly soluble with long-term stability, easily produced at high levels equivalent to those of therapeutic antibodies, and is amenable to conventional antibody purific

SUBMITTER: Mao C 

PROVIDER: S-EPMC11456856 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature