Snow alga Sanguina aurantia as revealed through de novo genome assembly and annotation.

ABSTRACT: To thrive on melting alpine and polar snow, some Chlorophytes produce an abundance of astaxanthin, causing red blooms, often dominated by genus Sanguina. The red cells have not been cultured, but we recently grew a green biciliate conspecific with Sanguina aurantia from a sample of watermelon snow. This culture provided source material for Oxford Nanopore Technology and Illumina sequencing. Our assembly pipeline exemplifies the value of a hybrid long- and short-read approach for the complexities of working with a culture grown from a field sample. Using bioinformatic tools, we separated assembled contigs into 2 genomic pools based on a difference in GC content (57.5 and 55.1%). We present the data as 2 assemblies of S. aurantia variants but explore other possibilities. High-throughput chro

SUBMITTER: Raymond BB 

PROVIDER: S-EPMC11457085 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature