Disrupting glioblastoma networks with tumor treating fields (TTFields) in in vitro models.

ABSTRACT:

Purpose

This study investigates the biological effect of Tumor Treating Fields (TTFields) on key drivers of glioblastoma's malignancy-tumor microtube (TM) formation-and on the function and overall integrity of the tumor cell network.

Method

Using a two-dimensional monoculture GB cell network model (2DTM) of primary glioblastoma cell (GBC) cultures (S24, BG5 or T269), we evaluated the effects of TTFields on cell density, interconnectivity and structural integrity of the tumor network. We also analyzed calcium (Ca²⁺) transient dynamics and network morphology, validating findings in patient-derived tumoroids and brain tumor organoids.

Results

In the 2DTM assay, TTFields reduced cell density by 85-88% and disrupted network interconnectivity, particularly in cel

SUBMITTER: Schlieper-Scherf S 

PROVIDER: S-EPMC11457690 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature