Host type 2 immune response to xenogeneic serum components impairs biomaterial-directed osteo-regenerative therapies.

ABSTRACT: The transformative potential of cells as therapeutic agents is being realized in a wide range of applications, from regenerative medicine to cancer therapy to autoimmune disorders. The majority of these therapies require ex vivo expansion of the cellular product, often utilizing fetal bovine serum (FBS) in the culture media. However, the impact of residual FBS on immune responses to cell therapies and the resulting cell therapy outcomes remains unclear. Here, we show that hydrogel-delivered FBS elicits a robust type 2 immune response characterized by infiltration of eosinophils and CD4⁺ T cells. Host secretion of cytokines associated with type 2 immunity, including IL-4, IL-5, and IL-13, is also increased in FBS-containing hydrogels. We demonstrate that the immune response to xe

SUBMITTER: Martin KE 

PROVIDER: S-EPMC11458135 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature