Development of a programmable automated cell culture system to study the lung pathophysiology of Cystic Fibrosis-related diabetes.

ABSTRACT: The study of many diseases is limited by the in vitro systems available. Cystic Fibrosis-Related Diabetes (CFRD), the main co-morbidity of Cystic Fibrosis (CF), is a perfect example. Cells in vivo experience glucose fluctuations after meals. In contrast, cells cultured in vitro are initially exposed to high glucose media. Glucose gets progressively depleted until the next media change days later, which is not physiologically relevant and could negatively impact the results of research studies. To better study the mechanisms driving CFRD pathophysiology, we developed a programmable and automated cell culture system (PACCS) capable of mimicking acute hyperglycemic episodes experienced by CFRD patients after meals. We adapted a commercially available perfusion system and

SUBMITTER: Vazquez Cegla AJ 

PROVIDER: S-EPMC11459049 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature