Nanomechanics of wild-type and mutant dimers of the inner-ear tip-link protein protocadherin 15.

ABSTRACT: Mechanical force controls the opening and closing of mechanosensitive ion channels atop the hair bundles of the inner ear. The filamentous tip link connecting transduction channels to the tallest neighboring stereocilium modulates the force transmitted to the channels and thus changes their probability of opening. Each tip link comprises four molecules: a dimer of protocadherin 15 (PCDH15) and a dimer of cadherin 23, all of which are stabilized by Ca²⁺ binding. Using a high-speed optical trap to examine dimeric PCDH15, we find that the protein's mechanical properties are sensitive to Ca²⁺ and that the molecule exhibits limited unfolding at a physiological Ca²⁺ concentration. PCDH15 can therefore modulate its stiffness without undergoing large unfolding even

SUBMITTER: Villasante CM 

PROVIDER: S-EPMC11459131 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature