Investigating the <i>cis-</i>regulatory basis of C₃ and C₄ photosynthesis in grasses at single-cell resolution.

ABSTRACT: While considerable knowledge exists about the enzymes pivotal for C₄ photosynthesis, much less is known about the cis-regulation important for specifying their expression in distinct cell types. Here, we use single-cell-indexed ATAC-seq to identify cell-type-specific accessible chromatin regions (ACRs) associated with C₄ enzymes for five different grass species. This study spans four C₄ species, covering three distinct photosynthetic subtypes: Zea mays and Sorghum bicolor (NADP-dependent malic enzyme), Panicum miliaceum (NAD-dependent malic enzyme), Urochloa fusca (phosphoenolpyruvate carboxykinase), along with the C₃ outgroup Oryza sativa. We studied the cis-regulatory landscape of enzymes essenti

SUBMITTER: Mendieta JP 

PROVIDER: S-EPMC11459142 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature