5-Hydroxymethylcytosine in circulating cell-free DNA as a potential diagnostic biomarker for SLE.

ABSTRACT:

Background

SLE is a complex autoimmune disease with heterogeneous manifestations and unpredictable outcomes. Early diagnosis is challenging due to non-specific symptoms, and current treatments only manage symptoms. Epigenetic alternations, including 5-Hydroxymethylome (5hmC) modifications, are important contributors to SLE pathogenesis. However, the 5hmC modification status in circulating cell-free DNA (cfDNA) of patients with SLE remains largely unexplored. We investigated the distribution of 5hmC in cfDNA of patients with SLE and healthy controls (HCs), and explored its potential as an SLE diagnosis marker.

Methods

We used 5hmC-Seal to generate genome-wide 5hmC profiles of plasma cfDNA and bioinformatics analysis to screen differentially hydroxymethylated regions (DhMRs).

SUBMITTER: Tong X 

PROVIDER: S-EPMC11459320 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature