Engineered pre-dentin with well-aligned hierarchical mineralized collagen fibril bundles promote bio-root regeneration.

ABSTRACT: Stem cell-mediated bio-root regeneration is an alternative tooth replacement strategy; however, physiologically functional bio-root regeneration with distinctive dentin structure remains challenging. In this study, the distinct arrangements of collagen fibril bundles were identified that account for hierarchical structural differences between dentin, cementum, and alveolar bone. Thus, an "engineered pre-dentin" was fabricated, which was a dentin hierarchical structure mimicking collagen (MC) scaffold, with well-aligned hierarchical mineralized collagen fibril bundles. The results revealed that it has a stronger effect on promoting biological root regeneration in nude mice and miniature pigs with dental pulp stem cell (DPSC) and periodontal ligament stem cell (PDLSC) sheets compared to hydr

SUBMITTER: Hu L 

PROVIDER: S-EPMC11459519 | biostudies-literature | 2024 Jan-Dec

REPOSITORIES: biostudies-literature