Chitinase 1: a novel therapeutic target in metabolic dysfunction-associated steatohepatitis.

ABSTRACT:

Background

Metabolic dysfunction-associated steatohepatitis (MASH) is characterized by persistent inflammatory cascades, with macrophage activation playing a pivotal role. Chitinase 1 (CHIT1), produced by activated macrophages, is a key player in this cascade. In this study, we aimed to explore the role of CHIT1 in MASH with progressive liver fibrosis.

Methods

Fibrotic liver tissue and serum from distinct patient groups were analyzed using nCounter MAX, flow cytometry, immunohistochemistry, and enzyme-linked immunosorbent assay. A MASH mouse model was constructed to evaluate the effectiveness of OATD-01, a chitinase inhibitor. Macrophage profiling was performed using single-nuclei RNA sequencing and flow cytometry.

Results

CHIT1 expression in fibrotic liver tissues wa

SUBMITTER: Cha JH 

PROVIDER: S-EPMC11459552 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature