Optimization, characterization and biosafety of carotenoids produced from whey using Micrococcus luteus.

ABSTRACT: This study aimed to optimize the production of carotenoid pigments from Micrococcus luteus (ATCC 9341) through the statistical screening of media components and the characterization of antimicrobial, antioxidant, cytogenetic and cytotoxic activities. A BOX-Behnken design was used to assess the effects of whey concentration, inoculum size, pH, temperature, and agitation speed on carotenoid yield. The optimum combination increased production to 2.19 g/L, with a productivity of 0.045 g L^-1 h^-1 and a productivity yield of 0.644 g/g, as confirmed by an observed carotene production of 2.19 g/L. The final response surface model fitting the data had an R² of 0.9461. High-performance liquid chromatography (HPLC) analysis identified 12 carotenoid pigment compounds pr

SUBMITTER: Hegazy AA 

PROVIDER: S-EPMC11459989 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature