NEK10 kinase ablation affects mitochondrial morphology, function and protein phosphorylation status.

ABSTRACT:

Background

NEK10, a serine/threonine/tyrosine kinase belonging to the NEK (NIMA-related kinases) family, has been associated with diverse cellular processes. However, no specific target pathways have been identified. Our previous work knocking down NEK10 in HeLa cells suggested a functional association with mitochondria, as we observed altered mitochondrial morphology, mitochondrial oxygen consumption, mtDNA integrity, and reactive oxygen species levels.

Methods

To better understand this association, we studied human HAP1 cells fully knockout for NEK10 and confirmed that NEK10 has an important role in mitochondrial homeostasis. We performed the study of mitochondrial respiration, mitochondrial morphology, mitochondrial mass, and mtDNA analysis. Additionally, we showed proteo

SUBMITTER: de Oliveira AP 

PROVIDER: S-EPMC11460017 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature