Dynamic landscape of m6A modifications and related post-transcriptional events in muscle-invasive bladder cancer.

ABSTRACT:

Background

Muscle-invasive bladder carcinoma (MIBC) is a serious and more advanced stage of bladder carcinoma. N6-Methyladenosine (m6A) is a dynamic and reversible modifications that primarily affects RNA stability and alternative splicing. The dysregulation of m6A in MIBC can be potential target for clinical interventions, but there have been limited studies on m6A modifications in MIBC and their associations with post-transcriptional regulatory processes.

Methods

Paired tumor and adjacent-normal tissues were obtained from three patients with MIBC following radical cystectomy. The additional paired tissues for validation were obtained from patients underwent transurethral resection. Utilizing Nanopore direct-RNA sequencing, we characterized the m6A RNA methylation landscape

SUBMITTER: Zhang L 

PROVIDER: S-EPMC11460118 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature