Proteomic analysis reveals molecular changes following genetic engineering in Chlamydomonas reinhardtii.

ABSTRACT:

Background

Chlamydomonas reinhardtii is gaining recognition as a promising expression system for the production of recombinant proteins. However, its performance as a cellular biofactory remains suboptimal, especially with respect to consistent expression of heterologous genes. Gene silencing mechanisms, position effect, and low nuclear transgene expression are major drawbacks for recombinant protein production in this model system. To unveil the molecular changes following transgene insertion, retention, and expression in this species, we genetically engineered C. reinhardtii wild type strain 137c (strain cc-125 mt+) to express the fluorescent protein mVenus and subsequently analysed its intracellular proteome.

Results

The obtained transgenic cell lines showed differences i

SUBMITTER: Barolo L 

PROVIDER: S-EPMC11460192 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature