Mapping Extracellular Protein-Protein Interactions Using Extracellular Proximity Labeling (ePL).

ABSTRACT: Proximity labeling (PL) has given researchers the tools to explore protein-protein interactions (PPIs) in living systems; however, most PL studies are performed on intracellular targets. We have adapted the original PL method to investigate PPIs within the extracellular compartment, which we term extracellular PL (ePL). To demonstrate the utility of this modified technique, we investigated the interactome of the matrisome protein TIMP2. TIMPs are a family of multifunctional proteins that were initially defined by their ability to inhibit metalloproteinases, the major mediators of extracellular matrix (ECM) turnover. TIMP2 exhibits broad expression and is often abundant in both normal and diseased tissues. Understanding the functional transformation of matrisome regulators, such as TIMP2, d

SUBMITTER: Peeney D 

PROVIDER: S-EPMC11460327 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature