<i>In situ</i> counter-diffusion crystallization and long-term crystal preservation in microfluidic fixed targets for serial crystallography.

ABSTRACT: Compared with batch and vapor diffusion methods, counter diffusion can generate larger and higher-quality protein crystals yielding improved diffraction data and higher-resolution structures. Typically, counter-diffusion experiments are conducted in elongated chambers, such as glass capillaries, and the crystals are either directly measured in the capillary or extracted and mounted at the X-ray beamline. Despite the advantages of counter-diffusion protein crystallization, there are few fixed-target devices that utilize counter diffusion for crystallization. In this article, different designs of user-friendly counter-diffusion chambers are presented which can be used to grow large protein crystals in a 2D polymer microfluidic fixed-target chip. Methods for rapid chip fabrication using comme

SUBMITTER: Liu Z 

PROVIDER: S-EPMC11460377 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature