Chirality governs the structure and activity changes of Photinus pyralis firefly luciferase induced by carbon quantum dots.

ABSTRACT: Nanobiocatalysis is a novel area integrating various advantages of nanotechnology and enzymatic catalysis. However, great efforts are still needed to fully understand the interactions between nanostructures and enzymes. The biological properties of nano-hybrid enzymes greatly depend on the size and chemical properties of their nano element. However, the impact of nanostructure chirality on the structure/function of the enzymes has not yet been fully investigated. In this study, using experimental and computational approaches, the interaction of Photinus pyralis firefly luciferase with chiral carbon quantum dots containing l and d-tryptophan constituent (l/d-Trp-CQDs) was investigated. Both the CQDs increased K _m of the enzyme for luciferin and resulted in the loss

SUBMITTER: Rad-Faraji M 

PROVIDER: S-EPMC11460436 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature