Screening and identification of linear B-cell epitopes on structural proteins of African Swine Fever Virus.

ABSTRACT: This study aims to screen and identify linear B-cell epitopes on the structural proteins of African Swine Fever Virus (ASFV) to assist in the development of peptide-based vaccines. In experiments, 66 peptides of 12 structural proteins of ASFV were predicted as potential linear B-cell epitopes using bioinformatics tools and were designed; the potential epitope proteins carried the GST tag were expressed, purified, and subjected to antigenicity analysis with porcine antiserum against ASFV, and further identified based on their immunogenicity in mice. A total of 22 potential linear B-cell epitopes showed immunoreactivity and immunogenicity. Of these epitopes, 13 epitopes were firstly identified including 4 epitopes located in p72 (352-363, 416-434, 424-439, 496-530 aa), 3 epitopes located in

SUBMITTER: Lu H 

PROVIDER: S-EPMC11460522 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature