Immunological responses to brain metastasis stereotactic radiosurgery in patient-matched longitudinal blood and tumour samples.

ABSTRACT:

Background

Stereotactic radiosurgery (SRS) is highly effective as focal treatment for brain metastases (BrMs), but whether it can promote anti-tumour immune responses that synergise with immunotherapy remains unclear. We investigated this by examining blood samples from a clinical trial for HER2-amplified breast cancer (HER2-BC) BrMs, matched with longitudinal HER2-BC BrM samples resected from the same location in the same patient.

Methods

Blood samples from 10 patients taken pre- and 7-14 days post-SRS were analysed by mass and flow cytometry. One patient received pre-operative SRS for a BrM that recurred 7 months after resection, followed by planned re-resection 8 days post-SRS. Pre- and post-SRS tumours from this patient were analysed by bulk RNAseq, multiplex immunohisto

SUBMITTER: Sia J 

PROVIDER: S-EPMC11460619 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature