Single-cell transcriptomic atlas of enteroendocrine cells along the murine gastrointestinal tract.

ABSTRACT:

Background

Enteroendocrine cells (EECs) produce over 20 gut hormones which contribute to intestinal physiology, nutrient metabolism and the regulation of food intake. The objective of this study was to generate a comprehensive transcriptomic map of mouse EECs from the stomach to the rectum.

Methods

EECs were purified by flow-cytometry from the stomach, upper small intestine, lower small intestine, caecum and large intestine of NeuroD1-Cre mice, and analysed by single cell RNA sequencing. Regional datasets were analysed bioinformatically and combined into a large cluster map. Findings were validated by L-cell calcium imaging and measurements of CCK secretion in vitro.

Results

20,006 EECs across the full gastrointestinal tract could be subdivided based on their full tra

SUBMITTER: Smith CA 

PROVIDER: S-EPMC11460673 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature