Tumour reoxygenation after intratumoural hydrogen peroxide (KORTUC) injection: a novel approach to enhance radiosensitivity.

ABSTRACT:

Background

KORTUC (0.5% hydrogen peroxide (H₂O₂) in 1% sodium-hyaluronate) releases cytotoxic levels of H₂O₂ in tissues after intratumoural injection. High levels of tumour control after radiotherapy plus KORTUC are reported in breast cancer patients. Here, we use human xenograft models to test the hypothesis that oxygen microbubbles released post-KORTUC are effective in modifying the hypoxic tumour microenvironment.

Methods and materials

Pimonidazole and Image-iT™ Red (live hypoxia marker) were utilised to assess dose-dependent changes in hypoxia post-H₂O₂ in HCT116 and LICR-LON-HN5 spheroids. Using a dual 2-nitroimidazole-marker technique and phospho-ATM we evaluated changes in hypoxia and reactive oxygen sp

SUBMITTER: Nimalasena S 

PROVIDER: S-EPMC11461272 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature